Optimal precursor ion selection for LC-MALDI MS/MS
نویسندگان
چکیده
منابع مشابه
Optimal precursor ion selection for LC-MS/MS based proteomics
Shotgun proteomics with Liquid Chromatography (LC) coupled to Tandem Mass Spectrometry (MS/MS) is a key technology for protein identification and quantitation. Protein identification is done indirectly: detected peptide signals are fragmented by MS/MS and their sequence is reconstructed. Afterwards, the identified peptides are used to infer the proteins present in a sample. The problem of choos...
متن کاملThe reproducibility of phospholipid analyses by MALDI-MSMS.
The identification of phosphocholine and phosphoethanolamine lipids by MALDI TOF/TOF, including characterisation of the headgroup and delineation of the acyl chain at each position of the glycerol backbone, has been explored using lipids representative of each type. The relative intensities of fragments involving the neutral loss of one or other of the acyl chains from ion adducts of 1-palmitoy...
متن کاملThe Power of LC MALDI: Identification of Proteins by LC MALDI
The Applied Biosystems 4700 Proteomics Analyzer with GPS ExplorerTM Software enables a fully automated and advanced LC MALDI MS/MS workflow for protein identification from complex mixtures. Novel MS peak detection identifies the presence of all peptides including those of closely related composition or isobaric peptides. Additionally, MS/MS spectra are collected at peak maxima increasing assay ...
متن کاملDetection of Crosslinks within and between Proteins by LC-MALDI-TOFTOF and the Software FINDX to Reduce the MSMS-Data to Acquire for Validation
Lysine-specific chemical crosslinking in combination with mass spectrometry is emerging as a tool for the structural characterization of protein complexes and protein-protein interactions. After tryptic digestion of crosslinked proteins there are thousands of peptides amenable to MSMS, of which only very few are crosslinked peptides of interest. Here we describe how the advantage offered by off...
متن کاملStructural Analysis of Triacylglycerols by Using a MALDI-TOF/TOF System with Monoisotopic Precursor Selection
A new MALDI-TOF/TOF system with monoisotopic precursor selection was applied to the analysis of triacylglycerols in an olive oil sample. Monoisotopic precursor selection made it possible to obtain product-ion mass spectra without interference from species that differed by a single double bond. Complete structure determination of all triacylglycerols, including structural isomers, was made possi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Bioinformatics
سال: 2013
ISSN: 1471-2105
DOI: 10.1186/1471-2105-14-56